Figure 2

Figure 2

Infection influences cell proliferation, and structure of collagen fibers in the regenerated caudal fin. (A) Blastema cellular proliferation after injection of PBS (CT) or LD, MD and HD infection with M. marinum prior to caudal fin amputation Anti-PH3 antibody staining of the cells in the fin at 6 hpA, expressed as fold change cut/uncut (mean ± SEM, n< 30, Kruskal-Wallis, Dunn’s multiple comparisons test, non-significant). (B) Blastema cell proliferation after injection of PBS (CT) or LD, MD and HD infection with M. marinum prior to caudal fin amputation. Anti-PH3 antibody staining of the cells in the fin at 24 hpA, expressed as fold change cut/uncut (mean ± SEM, n< 30, Kruskal-Wallis, Dunn’s multiple comparisons test, **p≤ 0.01, ***p≤ 0.001). (C) Second harmonic imaging Z projections were done after injection of PBS (CT) or LD, MD and HD of M. marinum at 72 hpA (Scale bar = 60 µm). The procedure of fiber alignment analysis is presented by representative images of the different conditions. The recorded second harmonic images (left) have been subjected to a fiber extraction algorithm (middle) and then the alignment of fiber endpoints (orientation of endpoints are marked with green lines) that were fit inside the fin-width-wide circular region of interests (ROI) (right) were analyzed. (D) Fiber alignment in the circular ROI after injection of PBS (CT) or infection with LD, MD or HD of M. marinum at 72 hpA (mean ± SEM, n<30, Kruskal-Wallis, Dunn’s multiple comparisons test, *p ≤ 0.05, **p ≤ 0.01).