IMAGE

Figure 7.

ID: ZDB-IMAGE-220404-27
Publication: Wiggin et al., 2022 - V3 Interneurons are Active and Recruit Spinal Motor Neurons During In Vivo Fictive Swimming in Larval Zebrafish
All Figures: Figures for Wiggin et al., 2022

Image

Figure Caption

Figure 7.

Spinal neurons loaded with rhodamine-2 AM dye are definitively identified as MNs. A, The EGFP-expressing parga^mn2Et enhancer trap line (A, green) was labeled with antibodies to choline acetyl transferase (ChAT; A′, magenta). Colabeling (A′′) of EGFP with ChAT antibodies indicated that EGFP expression in the parga^mn2Et line is a marker of spinal MNs. White arrowheads indicate ChAT-positive cells that do not contain EGFP (non-MNs). B, Live parga^mn2Et larvae were anesthetized, skinned, and incubated in rhodamine-2 AM dye (Rhod-2, AM). EGFP expression (B, green) and Rhod-2, AM labeling (B′, magenta), showed that Rhod-2, AM specifically labeled all MNs (B′′). White arrowheads indicate two Rhod-2, AM-labeled cells that did not contain EGFP. Scale bar: 20 μm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ eNeuro