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Calvird et al., 2022 - Uncovering Regulators of Heterochromatin Mediated Silencing Using a Zebrafish Transgenic Reporter

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FIGURE 1

The is7 transgene recapitulates key features of constitutive heterochromatin. (A–B) Brightfield (top) and fluorescence (bottom) images of representative wild-type (A) and is7/+(B) larva at 3 days post-fertilization (dpf). Scale bar indicates 1 mm. (C–D) Levels of the histone modifications H3K9me3, H4K20me3 and H3K27me3 at the is7 transgene array (C) or Sat1 pericentromeric repeats (D) as assessed by chromatin immunoprecipitation quantitative PCR (ChIP-qPCR). (E) 5-methycytosine (5 mC) enrichment (filled circles) at the is7 transgene array in two representative larval pools as detected by sodium bisulfite sequencing. Error bars indicate standard deviation.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol