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Fig. 2.
Vascular morphology is dependent on Wasb. (A) RT-PCR of 24 hpf FACS-processed ECs from Tg[fliep:eGFP]. Data are mean+s.e.m. (B) Trunk vasculature of 44 hpf Tg[fliep:nls-mCherry; fliep:lifeactGFP] F0 embryo injected with Cas9 mRNA (control) and Cas9 mRNA plus guide mRNA (wasb KO). (C) MO-control and MO-wasb Tg[fliep:nls-mCherry; fliep:lifeactGFP] embryos. Top panel of MO-wasb shows large vISVs with large diameter and aISVs with reduced diameter. Lower panel shows an example of a vascular malformation.
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