Fig. 1.
Pre-hypertrophic ceratohyal (ch) chondrocytes express entpd5a:kaede soon after differentiation. Confocal images of live double-transgenic embryos. (A-D) sox10:lyn-tdtomato;entpd5a:kaede double-transgenic embryos at 72 hpf (A,B) and 96 hpf (C,D). (E-L) col10a1a:Citrine;entpd5a:KillerRed double-transgenic embryos at 78 hpf (E-H) and 144 hpf (I-L). (E,I) Optical slices in DIC. (A,C,E,I) Optical slices. (B,D,F-H,J-L) z-projections. White arrowheads indicate the position of the pre-HZ. BR3, branchiostegal ray 3; ch, ceratohyal; D, dorsal; pq, palatoquadrate; V, ventral. All micrographs are ventral views with anterior to the left. Scale bar: 50 ?m (A-L).