FIGURE 4

FIGURE 4

Analysis of rhNGF-induced cell proliferation upon light-induced degeneration (LID). (A–J) Representative images of retinal cryosection of adult zebrafish eyes immunostained with anti-PCNA antibody, which labels proliferating cells (green), and stained with DAPI (blue). (A_I–J_I) Images extrapolated from A–J displaying exclusively the PCNA signal. (A,A)_I Retinal cryosection of an adult zebrafish eye not exposed to light (N retinae: 9). (B,B_I) Retinal cryosection of adult zebrafish eye exposed to 60 h of LID (N retinae: 9). (C,C_I) Cryosection of untreated adult zebrafish retina at 7 dpi (N retinae: 10). (D,D_I) Retinal cryosection of rhNGF-injected adult zebrafish eye at 7 dpi (N retinae: 10). (E,E_I). Cryosection of untreated adult zebrafish retina at 14 dpi (N retinae: 9). (F,F_I) Retinal cryosection of rhNGF-injected adult zebrafish eye at 14 dpi (N retinae: 9). (G,G_I) Cryosection of untreated adult zebrafish retina at 21 dpi (N retinae: 10).(H,H_I) Retinal cryosection of rhNGF-injected adult zebrafish eye at 21 dpi (N retinae: 8). (I,I_I) Cryosection of untreated adult zebrafish retina at 28 dpi (N retinae: 9). (J,J_I) Retinal cryosection of untreated rhNGF-injected adult zebrafish eye at 28 dpi (N retinae: 9).(K) Quantification of proliferating cells. Ratio of PCNA-positive cells over the analyzed area is shown. Data are shown as means ± SEM (n = 10). ***p < 0.001; **p < 0.01; *p < 0.05 (one-way ANOVA test followed by a Sidak’s multiple comparison test); 0 h LID T 7 dpi vs. 60 h LID T 7 dpi + rhNGF: Adjusted p-value:< 0.001; 60 h LID T 14 dpi vs. 60 h LID T 14 dpi + rhNGF: Adjusted p-value: 0.0379; 60 h LID T 21 dpi vs. 60 h LID T 21 dpi + rhNGF: Adjusted p-value: 0.0412. DPI: days post injury. Scale bar: 50 μm.