FIGURE 2

FIGURE 2

Light-induced retinal degeneration zebrafish model. (A,B) Representative images of retinal cryosections of adult zebrafish eyes immunostained with Zpr1 antibody (red), which labels photoreceptor cells, and stained with DAPI (blue), which labels nuclei. (A) Retinal cryosection of adult zebrafish eye not exposed to constant light (NO LID). (B) Retinal cryosection of adult zebrafish eye exposed to 60 h of constant light (light-induced degeneration—LID). (C) Quantification of ONL thickness (number of retinae analyzed = 12). (D) Quantification of ONL cell number (number of retinae analyzed = 12, total number of cells: 305 for injured and 1,243 for control retinae). (E,F) Representative images of retinal cryosections of adult zebrafish eyes stained with TUNEL (red), which labels apoptotic cells, and with DAPI (blue), which labels nuclei. (E) Retinal cryosection of adult zebrafish eye not damaged by light exposure (NO LID). (F) Retinal cryosection of adult zebrafish eye after 60 h of LID. (G) Quantification of TUNEL-positive cells shown as mean number per retina (number of retinae analyzed = 12). Data are shown as means ± SEM. ***p < 0.001 (Mann–Whitney test). Scale bar: 50 μm.