Figure 7

Figure 7

(A) 60-hpf wild-type and strip1^rw147 mutants combined with the transgenic line Tg[hsp:mCherry-Bcl2]. Nontransgenic embryos (Bcl2−, top panels) are compared to transgenic embryos (Bcl2+, bottom panels) after heat shock treatment. Apoptotic cells are visualized by transferase dUTP nick end labeling (TUNEL) FL and fluorescent signals from mCherry-Bcl2 are shown. Nuclei are stained with Hoechst. (B) The number of TUNEL+ cells in ganglion cell layer (GCL). Two-way analysis of variance (ANOVA) with the Tukey multiple comparison test, n = 3. (C) 78-hpf wild-type and strip1^rw147 mutant retinas combined with the transgenic lines, Tg[ath5:GFP] and Tg[hsp:mCherry-Bcl2]. Nontransgenic embryos (Bcl2−, top panels) are compared to transgenic embryos (Bcl2+, bottom panels) after heat shock treatment. RGCs are labeled with ath5:GFP and fluorescent signals from mCherry-Bcl2 are shown. Anti-acetylated α-tubulin labels the IPL. Nuclei are stained with Hoechst. Arrowheads represent areas where RGC dendrites contribute to the IPL. Asterisks denote areas where RGC dendrites fail to project to the forming IPL and a fraction of presumptive amacrine cells is located between them. (D) Percentage of ath5+ area relative to retinal area. Two-way ANOVA with the Tukey multiple comparison test, n ≥ 3. Scale bar, 50 μm (A, C). For all graphs, data are represented as means ± standard deviation (SD). ns, not significant, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.

Figure 7—source data 1.