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Figure 5—figure supplement 1.

ID: ZDB-IMAGE-220324-45
Source: Figures for Saraswathy et al., 2022

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Figure Caption

Figure 5—figure supplement 1. Mib1 is dispensable for Wnt5b-induced Ryk degradation.

(A,B) 17 out of 18 Wnt5b-injected embryos present severely reduced Ryk-GFP signals compared to WT controls (n = 12). (C,D) A similar Wnt5b-dependent decrease of Ryk-GFP levels is observed in mib1^tfi91 homozygous mutants that were additionally injected with dominant-negative Mib1ΔRF123 (n = 14 mutant and n = 16 WT control embryos). All pictures depict dorsal views of 90% epiboly stage embryos, anterior up. The Histone2B-mRFP signal (A’-D’) was used to ascertain that control and Wnt5b-expressing embryos had received comparable amounts of injected material. Scalebars: 10 µm.

Acknowledgments

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