IMAGE

Fig 7

ID

ZDB-IMAGE-220319-56

Genes

EGFP
opn1mw1

Antibodies

Ab3-opn1mw1

Publication

Liu et al., 2022 - Rod genesis driven by mafba in an nrl knockout zebrafish model with altered photoreceptor composition and progressive retinal degeneration

All Figures

Figures for Liu et al., 2022

Image

Figure Caption

Fig 7 Single-cell RNA-seq analysis in WT and <italic toggle='yes'>nrl</italic>-KO retinas.

(A) tSNE visualization of the unsupervised cell clusters from 5-month-old WT and nrl-KO zebrafish. Left, the distribution of cell clusters between the WT and nrl-KO groups. Right, the retinal cell types identified via scRNA-seq (see also S6 Fig). BC, bipolar cells; Cone_R/G, red and green cones; Cone_B/UV, UV and blue cones; RGC, retinal ganglion cells; HC, horizontal cells; AC, amacrine cells; RPE, retinal pigment epithelium. (B) Proportions of each retinal cell type in the WT and nrl-KO zebrafish. (C) The cell compositions and proportions of photoreceptor sub-clusters in WT and nrl-KO retinas. (D) The heatmap shows the clustering pattern of the top 20 down-regulated and top 20 up-regulated genes in the WT and nrl-KO rods. Yellow, high expression; purple, low expression. (E) Mis-expression of green-cone opsin in a proportion of rods in the nrl-KO retinas. The dorsal retinal regions of WT and nrl-KO zebrafish at 3 mpf are shown. Green, EGFP-labeled rods. Red, immunofluorescence signals of the anti-Opn1mw antibody. The green fluorescent signal in the nrl-KO group was artificially enhanced to discern the morphology of rods. ROS, outer segments of rods; GCOS, outer segments of green cones; RN, rod nuclear layer. Scale bar: 20 μm.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.