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Figure 4
Figure 4. Analysis of crosstalk between GR and Stat3 with zebrafish mutant and transgenic lines. (A) Representative pictures (A) and fluorescence quantification (A’) of Tg(7xStat3-Hsv.Ul23:EGFP)ia28 6 dpf larvae at the level of the intestine in gr+/+1, gr+/ia30 and gria30/ia30 genetic background. Scale bar= 100 μm. (B) Representative pictures (B) and fluorescence quantification (B’) of Tg(7xStat3-Hsv.Ul23:EGFP)ia28 6 dpf larvae at the level of the intestine in gr+/+2, gr+/s357 and grs357/s357 genetic background. Scale bar = 100 μm. (C) Representative pictures (C) and fluorescence quantification (C’) of Tg(9xGCRE-Hsv.Ul23:EGFP)ia20 incubated from 3 to 6 dpf with DMSO, 50 μM AG490 and 20 μM LIF. Scale bar = 500 μm. (D) representative pictures of 6 dpf larvae generated by the breeding between gr+/ia30/stat3+/ia23 zebrafish. Scale bar = 500 μm. (E) Table of observed (OV) and expected (EV) values of animals belonging to the 9 different genotypes obtained from breedings between gr+/ia30/stat3+/ia23 zebrafish: χ2 test shows not significant differences between OV and EV in genotype distribution until 72 hpf (p-value = 0.7196); significant differences between OV and EV were detected at 4 dpf (** p-value = 0.0058) and 3 mpf (**** p-value = 4.30949 × 10−22). Mean ± SEM. Statistical analyses were performed with Student’s t test (A,B,C) and χ2 test (E). * p < 0.05; ** p < 0.01; *** p < 0.001; ns = not significant.
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