Figure 4

Figure 4

Analysis of the spatial YCL asters distribution in rings. (A) Maximum projection of a dclk2-GFP embryo where 22 YCL asters can be identified (yellow crosses) distributed in four rings. Identifiers 1 and 2 correspond to the vegetal pole and to the center of the embryo, respectively. (B) Projection of the aster position over the sphere modeling the yolk. Asters belonging to the same ring are represented by dots of the same color, rings are highlighted as belts at the calculated latitude. (C) YCL asters latitude (polar angle). YCL asters are not randomly located, but they group at four different latitudes (rings) (blue, red, green, and yellow). (D) Azimuthal angle differences between neighboring asters in rings 1 (blue), 2 (red), 3 (green), and 4 (yellow). Asters in this embryo are equidistant in 6/9 cases in ring1 and 4/6 cases in ring2. Cases with higher distances are marked with asterisks. (E–H) The same analysis for an embryo with only one YCL asters ring. Here 5 YCL asters can be identified: 4 asters distributed around 360°, and one in the vegetal pole. Each aster is highlighted with a yellow cross in Fig. 3E. Identifiers 1 and 2 correspond to the vegetal pole and to the embryo center, respectively. For this particular case, asters are equidistant (H). (I) Plot of the distribution of YCL asters in 1, 2, 3, or 4 rings. The analysis was performed on 40 embryos. (B,F) Created with MATLAB R2020b (https://www.mathworks.com).