Figure 5

Figure 5. FO is not related to estrogenic activity in ovariectomy (OVX)-induced mice and estrogen response element (ERE)-luciferase transfected MCF-7 cells. (A) OVX-induced mice were orally fed with 100 mg/kg and 200 mg/kg FO and intraperitoneally (IP) injected with 10 ?g/kg 17?-estradiol (E2) for 4 weeks. After termination, uterine weight was determined by calculating uterine weight in body weight. (B?D) MCF-7 breast cancer cells (1 × 10⁴ cells/mL) transfected with human estrogen response element (ERE)-luciferase reporter plasmid for 24 h and (B) concentration?response curve of transcriptional activation for FO (2 × 10^?4?2 × 10² ?g/mL), corticosterone (10^?11?10^?5 M), genistein (10^?10?10^?4 M), and E2 (10^?14?10^?8 M) were measured using luciferase activity. (C,D) In a parallel experiment, FO (200 ?g/mL) and E2 (10^?9 M) were treated in the presence or absence of 10 ?M ICI 182,780 (ICI), and (C) relative luciferase activity and (D) relative cell proliferation were calculated. All data are presented as means ± standard error of the mean (and ^### p < 0.001 and ^## p < 0.01 vs. untreated MCF-7 cells; *** p < 0.001 and ** p < 0.01 vs E2-treated MCF-7 cells).