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Figure Caption
Fig. 1 Generation of the lepr knockout (lepr−/−) line. (A) CRISPR/Cas9 was used to target a 20-base pair (bp) region of exon eight in the zebrafish lepr gene. (B) The frameshifted lepr mutant yielded a truncated protein, due to an additional region of altered translation in which a stop codon was generated upstream the functionally important Ig-like C2-type domain of the lepr gene. The domains were estimated from human LEPR (GenBank Acc. No. AAA93015).
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Full text @ Gen. Comp. Endocrinol.