ZFIN Image: Marquez-Legorreta et al., 2022, Fig. 5

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Figure Caption

Fig. 5

Behavioral and network-wide changes in fmr1⁻^/⁻ larvae.

a Probability of response across the three groups to two blocks of ten looms. Over the course of two blocks of 10 stimuli, fmr1⁻^/⁻ larvae show slower habituation and stronger recovery than WT siblings, and heterozygotes show an intermediate phenotype. One-sided binomial test: fmr1⁻^/⁻ versus WT: 2nd Loom (p = 3.056e⁻⁵); 3rd Loom (p = 0.034); 11th Loom (p = 0.055); 16th Loom (p = 0.039). Heterozygotes versus WT: 2nd Loom (p = 0.001) and 9th Loom (p = 0.039). All other comparisons (p > 0.1). Significance cutoffs using a Bonferroni correction are *p < 0.00125 and **p < 0.00025, equivalent to uncorrected p < 0.05 and 0.01, respectively. b Brain-wide distributions of ROIs for the three genotypes, color-coded for functional cluster as in Figs. 2 and 3a. A random sample (n = 11) of Hets was selected to match WT (n = 10) and fmr1⁻^/⁻ (n = 11). c Node-based graphs showing relative correlations (WT correlation minus fmr1⁻^/⁻ correlation), where blue indicates correlations that are stronger in WT and red indicates correlations that are stronger in fmr1⁻^/⁻. Larvae imaged to generate the graphs for WT = 10 and fmr1⁻^/⁻ = 11. d Heat maps of participation for all nodes across habituation and recovery. e A raster plot of relative participation (WT participation minus fmr1⁻^/⁻ participation) for each node through the first 11 trials.

Acknowledgments

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