Fig. 4
Swimming patterns of larvae and adults in response to test compounds. The locomotion tracking plots (or ‘traces’) extracted from wrMTrck for A untreated, B scopolamine (SCOP)-treated, C isoxazole (ISOX)-treated, D ISOX + SCOP-treated, E Convolvulus pluricaulis (CP)-treated, and F CP + SCOP-treated larvae. The 168 hpf larvae were treated with the least toxic concentrations of SCOP (6.68 μM), ISOX (1.04 mM), and CP (0.38 mg/mL) and their combinations. Note that each larva is represented by a single trace. Three independent experiments were conducted with each of the treatment conditions to determine the locomotion patterns of the larvae. Three minutes long videos (2 min for acclimatization and 1 min for test response) of 3 larvae (per Petri dish placed on a light source) were shot in a dark chamber