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FIGURE 2
FRET-based detection of drug-induced cancer cell apoptosis in vitro.(A) Principle of caspase-3 reporter Sensor C3. (B) Observation of cell apoptosis in A549-C3 cells treated with 10 µM DOX for 9 h. CFP (ex: 430 nm/em: 480 nm) was merged with YFP (ex: 430 nm/em: 520 nm). Blue cells in merged images indicate apoptotic cells. (C) Time course and dose-dependent detection of cell apoptosis in 231-C3 cells treated with cisplatin. Blue cells in merged images indicate apoptotic cells. Scale bar = 50 µm.
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Full text @ Front Bioeng Biotechnol