IMAGE

Fig. 7.

ID

ZDB-IMAGE-220304-8

Antibodies

Publication

Allanki et al., 2021 - Interleukin-11 signaling promotes cellular reprogramming and limits fibrotic scarring during tissue regeneration

All Figures

Figures for Allanki et al., 2021

Image

Figure Caption

Fig. 7. Il-11 signaling in endothelial cells allows CM repopulation after cardiac injury.

(A) Experimental design and confocal images of immunostaining (Aldh1a2, magenta; αSMA, yellow) on cryosections from vehicle-treated il11ra^+/− siblings (n = 6) and vehicle- or 4-OHT–treated il11ra^−/− (n = 4 and 5, respectively) Tg(fli1:CreER); Tg(hsp70l:LBL-il11ra-p2a-mCh) ventricles at 7 dpci. (B and C) Quantification of total αSMA⁺ cell density (B) and αSMA⁺ Aldh1a2⁺ cell density (C) in the injured area, 7 dpci. (D) Experimental design and F-actin staining on 50-μm-thick cryosections from vehicle-treated il11ra^+/− siblings and vehicle- or 4-OHT–treated il11ra^−/− Tg(fli1:CreER); Tg(hsp70l:LBL-il11ra-p2a-mCh) ventricles at 7 dpci. (E and F) Quantification of the number per ventricle (E) (il11ra^+/− veh, n = 6; il11ra^−/− veh, n = 4; il11ra^−/− 4-OHT, n = 5) and length (F) (il11ra^+/− veh, n = 481; il11ra^−/− veh, n = 185; il11ra^−/− 4-OHT, n = 437) of CM protrusions at 7 dpci. Data represent means ± SD (B, C, E, and F). One-way ANOVA (B, C, and E); Kruskal-Wallis test (F). n, ventricles (A and E); n, CM protrusions (F). Yellow dashed lines demarcate the injured area (A and D); white arrowheads point to αSMA⁺ Aldh1a2⁺ endocardial cells (A, insets). Scale bars, 50 μm (A and D) and 10 μm (A, insets).

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci Adv