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Fig. 4 Inhibition assay of zebrafish Neu3.2 and Neu3.3. Sialidase activity of Neu3.2 (A) and Neu3.3 (B) was measured using 4MU-NANA as substrate (control) and in presence of two concentrations of the competitive inhibitor DANA. The kinetic parameters Km and Vmax for the artificial fluorescent substrate of the two paralogs and the corresponding Ki values are reported in C and D, respectively.
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