ZFIN Image: Torregrosa-Carrión et al., 2021, Fig. 2.

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Figure Caption

Fig. 2.

Conditional Gpr126 deletion in either the endocardium or all cardiac tissues allows embryonic survival.

(A to D″) H&E staining of transverse heart sections from WT (A to A″), Gpr126^fl/fl;Nfatc1^Cre/+ (B to B″), Gpr126^fl/fl;Tie2^Cre/+ (C to C″), and Gpr126^fl/fl;Mesp1^Cre/+ (D to D″) embryos at E15.5. Magnified views of the right ventricle are shown in (′) and of the left ventricle in (″). (E) qRT-PCR analysis showing relative Gpr126 gene expression (to Gapdh) in Gpr126^fl/fl;Mesp1^+/+ and Gpr126^fl/fl;Mesp1^Cre/+ embryonic hearts at E12.5 using primers spanning exons 4 to 7 and exons 9 and 10. Data are means ± SD (n = 3 embryonic hearts); t test, *P < 0.05 and ***P < 0.001. (F to H) H&E staining of transverse heart sections from WT Gpr126^fl/Δ7 (F), Gpr126^fl/Δ7;Tie2^Cre/+ (G), and Gpr126^fl/Δ7;Mesp1^Cre/+ (H) embryos at E16.5. Left panels: Right ventricle; Right panels: Left ventricle. (I) Schematic of the conditional gain-of-function R26-GPR126^GOF construct. GPR126 expression is activated upon the Cre-mediated excision of the NeoR-STOP cassette. (J) Top panels: Confocal images showing eGFP expression in E9.5 GPR126^GOF/+ and GPR126^GOF/+;Nkx2.5^Cre/+ hearts. Bottom panels: WISH showing GPR126 expression in E9.5 GPR126^GOF/+ and GPR126^GOF/+;Nkx2.5^Cre/+ hearts. (K) Representation of GPR126^GOF/+;Gpr126^Δ7/Δ7;Tie2^Cre/+ embryos from crosses of Gpr126^Δ7/+;Tie2^Cre/+ males with GPR126^GOF/+;Gpr126^Δ7/+ females. Scale bars, 100 μm. GFP, green fluorescent protein; IRES, internal ribosome entry site; mv, mitral valve; neoR, neomycin resistance gene; R26 pr, Rosa26 promoter; tv, tricuspid valve.

Acknowledgments

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