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Publication: DiNapoli et al., 2022 - Histone 3 Methyltransferases Alter Melanoma Initiation and Progression Through Discrete Mechanisms
All Figures: Figures for DiNapoli et al., 2022

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FIGURE 2

H3.3K9M induces interferon response. (A) Western blot of acid-extracted histones in A375 cells expressing H3.3 WT or H3.3K9M transgenes. (B) Percentage of mScarlet+ cells sorted from a heterogeneous H3.3-mScarlet and H3.3K9M-mScarlet cell population using non-transfected cell as mScarlet control. (C) MA plot of H3.3K9M vs H3.3 (Peaks that change at FDR <0.01 are colored red). (D) Top 1000 differentially expressed genes between H3.3-mScarlet and H3.3K9M-mScarlet (sorted by p-value adjusted). (E) Plot displaying Enriched signatures in H3.3K9M expressing A375 cells. Plot generated by GO Biological Process 2018 Enrichr (Mi et al., 2013). (F) ATAC-seq peaks that are differentially more accessible in H3.3K9M cells. (G) RNA seq normalized counts for CD33 in sorted cells (n = 4), (p < 0.0001).

Acknowledgments

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