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Figure 3
miR-204-5p mRNA expression levels quantified by RT-PCR during (A) embryogenesis (3, 7 and 14 days post fecundation (dpf)) and (B) aging (2 and 6 months, 1 and 2 years old) in a zebrafish larvae in vivo model. (C) ERK, pERK and aggrecan protein levels assessed by western blot (top) and quantified as a relative optical density ratio vs. B-actin (down) on protein lysates from a zebrafish larvae in vivo model. (* p < 0.05; ** p < 0.005 vs. expression levels of larvae after 3 (dpf) (A,B) and vs. 1 year old (C)).
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Full text @ Int. J. Mol. Sci.