ZFIN Image: Fujii et al., 2022, Figure 2

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Figure 2

Quantification of total post-translational modifications in type I collagen secreted from ZF4 cells. Culture media of ZF4 cells grown at 4 °C was pepsin-treated, and collagen was purified through salt precipitation. Purified collagen was subjected to SDS-PAGE and transferred to a PVDF membrane. Then, ?1(I) + ?3(I) and ?2(I) bands were excised and subjected to acid hydrolysis with SI-collagen. Pro, Lys, 3-Hyp, 4-Hyp, and total Hyl (Hyl + glycosylated Hyl) were quantified using LC-MS. Values for 4-Hyp (4-Hyp/(Pro + 4-Hyp + 3-Hyp)), total Hyl (total Hyl/(Lys + total Hyl)), and 3-Hyp (3-Hyp/(Pro + 4-Hyp + 3-Hyp)) ratios were calculated. Values are means ± SD (n = 3). Differences of means were assessed by one-way ANOVA followed by a Tukey?s post-hoc test.

Acknowledgments

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