Figure 4

Behavioral antiseizure analysis of the R- and S-enantiomers of halimide in the zebrafish PTZ seizure model. (A) Chemical structures of (S)-halimide and (R)-halimide. (B–E) Antiseizure activity of (S)-halimide (B,C) and (R)-halimide (D,E) in the zebrafish pentylenetetrazole (PTZ) seizure model after 2 h of incubation. Behavioral data is expressed in mean actinteg units per 5 min (±SEM) during the 30 min recording period (B,D) and over consecutive time intervals (C,E). Data is normalized against the vehicle (VHC) + PTZ control condition (C,E). Data are pooled from two independent experiments with a total of 20 replicate wells for VHC + VHC, VHC + PTZ, and compound + PTZ conditions, and 11–12 replicate wells for compound + VHC conditions. Statistical analysis: (B,D) one-way ANOVA with Dunnett’s multiple comparison test, (C,E) two-way ANOVA with Dunnett’s multiple comparison test (GraphPad Prism 9, San Diego, CA, USA). Significance levels: * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001.