ZFIN Image: Paulissen et al., 2022, Figure 3

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Figure Caption

Figure 3

The somitic mesoderm, not the notochord, is required for midline convergence of angioblasts.

(A) Time-lapse imaging of tg(kdrl:eGFP) embryos injected with control morpholino over a 240′ period. (B) tg(kdrl:eGFP) embryos injected with noto morpholino over a 240′ period. Red arrows indicate the midline. (C) Graph of embryos with midline angioblasts in noto and control morphants after 240′. N = 15 for both conditions (D) Time-lapse imaging of wild-type, tg(kdrl:eGFP) embryos over a 240′ period. (E) Time-lapse imaging of tbx16-/-, tg(kdrl:eGFP) embryos over a 240′ period. Red arrows indicate the midline. (F) Graph of embryos with midline angioblasts in wild-type and tbx16-/- after 240′. N = 18 for both conditions. (G) Section of wild-type, tg(kdrl:eGFP) embryos over a 240′ period. (H) Section of 24 hpf tg(kdrl:eGFP) embryos injected noto morpholino and stained with DAPI. (I) Section of 24 hpf tg(kdrl:eGFP) embryos injected tbx16 morpholino. Blood vessels are labeled in green and nuclei labeled in gray. Scale bars, 50 µm.

Acknowledgments

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