ZFIN Image: Janiak et al., 2022, Fig. 7

Image

Figure Caption

Fig. 7

2P plane-bending to image the in vivo larval zebrafish brain.

a–c Schematic of HuC:GCaMP6f larval zebrafish brain viewed from top (a) and front (b) with scan planes indicated, and (c) example-scan-profiles. d nTC₁ 512 × 1024 scans of a 6 dpf zebrafish brain with different plane curvatures, with peak axial displacement at scan centre as indicated. At curvatures ~100–150 µm peak displacement the scan approximately traverses the surface of the tectum. e–h Mean (e), activity-correlation (f, cf. Fig. 3c) and fluorescence traces (g, raw and h, event-triggered mean) from a 170 × 340 px scan (5.88 Hz) of the 100 µm peak displacement configuration (image 3 in (d)). The fish was presented with full-field and spectrally broad (~360–650 nm) series of light-flashes. See also Fig. ^S4.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.