Fig. 3

Fig. 3

p53 is not required for the senescence phenotype. (A) Immunoblots show the expression of indicated proteins of Mel202 cells infected by vector control (vec) or BAP1‐targeting sgRNAs (g1, g2, and g3). The blots shown are representative of three independent experiments. (B) Growth curve of Mel202 and Mel202 p53 KO clones (#1 and #2) infected by vector control (vec) or BAP1‐targeting g2 sgRNA. Immunoblots show BAP1 KO efficiency at the final time point (12 days) in Mel202 and Mel202 p53 KO clones (#1 and #2). The data are presented as the mean ± SD (n = 3) from one representative experiment out of three. (C) Colony‐formation assay of Mel202 and Mel202 p53 KO clones (#1 and #2) infected by vector control (vec) or BAP1‐targeting g2 sgRNA, and the colonies were stained with crystal violet for quantification. The data are presented as the mean ± SD (n = 3) from one representative experiment out of three. Student’s t test was used for statistical analysis.