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Fig. 2. In vitro anti-mycobacterial activity of nNF derivatives against extracellular and intracellular Mm. (A) The nitroaromatic backbone structure of the nNF compounds tested in this study. (B) Activity of nNF derivatives against extracellular Mm determined by MIC90. (C) Anti-mycobacterial activity of nNF derivatives and RIF against intracellular Mm in murine BMDMs. BMDM cultures were infected with Mm at 32.5°C at a MOI of 0.1 and treated with nNF derivatives at different concentrations based on their MIC90. Intracellular mycobacterial burden was determined by CFU plating at 4 days post infection. Data represent the meanąs.d. of three independent experiments. nd, not detected.