ZFIN Image: Zhou et al., 2021, Fig. 4

Image

Figure Caption

Fig. 4 CRHR mediates the effects of CRH on zebrafish ovary. (A and B) Follicular cells were treated with or without CRHR inhibitor (?-helical 9?41) (10 ?M) for 1 h, followed by 100 nM CRH? or 100 nM CRH? treatment for 5 min and 10 min, respectively. Representative blots and quantified graphs (n = 3) are shown. (C and D) Follicular cells were treated with or without CRH? or CRH? (100 nM) in the presence or absence of ?-helical 9?41 for 24 h. The mRNA expression of cyp19a1a, hsd17b1, and hsd17b3 were detected using qPCR (n = 4). The content of E2 in the medium was measured by ELISA (n = 4). Different lowercase above columns indicates statistical significance (P < 0.05).

Acknowledgments

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Reprinted from Molecular and Cellular Endocrinology, 527, Zhou, H., Chen, A., Lu, W., Corticotropin-releasing hormone reduces basal estradiol production in zebrafish follicular cells, 111222, Copyright (2021) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.