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Fig. 6 Loss of the TMD reduces expression of otoferlin in cell culture. (A) Representative images of cells transfected with CFP-VAMP and either WT mouse otoferlin or ΔTMD, a mouse otoferlin with a mutation matching the otofB mutation. (B) Representative images of cells labeled with 4′,6-diamidino-2-phenylindole (blue), wheat germ agglutinin for the cell membrane (red), and otoferlin (green). (C) Quantitation of the intensity of otoferlin from transfected HEK cells (t test, p < 0.001). Scale bar for A and B = 2 µm, and 10 µm for C and D.
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