Species tree topology used for evolutionary analyses (A), and branch specific Ka/Ks ratios obtained from different data sets (B?D). Each of the three electric fish clades was labeled as follows: a-Mormyroidea, b-Malapterurus electricus, and c-Gymnotiformes; and nonelectric taxa used for comparisons are labeled as follows: d-Asian arowana, e-Channel catfish, and f-Cave fish. Images of representative electric fishes are provided (A). The Ka/Ks ratios for each terminal branch were calculated from all 4,657 orthologs (B), concatenated all orthologs (C), and 1,000 concatenated alignments constructed from ten randomly chosen orthologs (D).