ZFIN Image: Tesmer et al., 2022, Fig. 7

Image

Figure Caption

Fig. 7

Visual receptive fields of PyrNs, SMTLs, and RGCs. A Overview of experimental design to monitor ramp-evoked responses in elements of the tectum-TL circuit. Left and right displays are used to present single 15° squares within a 6x4 checkerboard in a pseudorandom pattern. GCaMP6s signal intensity was used to quantify response strength at each position. Position response strength was subsequently used to generate RF maps. B Maximum projection image of an image timeseries acquired in tectum of a id2b:gcamp6s larva presented with RF mapping stimuli. C Example RF maps for the four PyrNs indicated in B. Note large, complex RFs and asymmetries in RF size between left and right visual fields. D Quantification of RF size in contralateral and ipsilateral RFs of individual PyrNs. Note high degree of variability in RF size and contra/ipsi ratio. E Distribution of contra/ipsi RF size ratio for 55 PyrNs recorded in 7 larvae. F Comparison of RF size in PyrNs, SMTLs, and RGCs. One-way ANOVA with Tukey?s multiple comparisons test, p<0.0001 for comparisons between each indicated group and the contra-PyrN group. G Maximum projection image of an image timeseries acquired in TL of a gff23c:gcamp6s larva presented with RF mapping stimuli. Arrow denotes SMTL with visible axon extending towards the right tectal lobe. H Example RF maps for the four SMTLs indicated in G. Note large, but discrete RFs and asymmetries in RF size between left and right visual fields. I Maximum projection image of an image timeseries acquired in the left tectum of a atoh7:gcamp6s larva presented with RF mapping stimulus to the right eye. Overlaid on this image are active RGC ROIs detected in response to presentation of a 30s luminance ramp stimulus to the right eye. Arrows denote four RGC ROIs at distinct positions along the A?P axis. J Example RF maps for the four RGC ROIs indicated in I. Note compact, discrete RFs and nasal-temporal distribution of RF positions match anteroposterior position of ROIs in the tectum. Scale bar: 75?m in B, 25?m in G, and 40?m in I

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Biol.