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Figure 3
Main hallmarks of cell proliferation and cell apoptosis in liver tissues from twist1a+/kras+ double transgenic zebrafish. (A) Immunohistochemical staining micrograph showing proliferating and apoptotic cells in liver cross-sections from wild-type, twist1a+, kras+, and twist1a+/kras zebrafish. (B) Quantification of the percentage of positive cells for the all fields following PCNA and caspase-3 staining at 4 wpi using ImageJ. Scale bar: 50 μm. Student’s t-tests were used to assess differences between variables: ** p < 0.01, *** p < 0.001.
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