|
Fig. 2 Model illustrating the fin movement of different pax3 and pax7 mutants. A, Embryos belonging to group A have the capability of moving their pectoral fins both away and toward the body, they can also move their pectoral fin in an angle >90° away from the body, group A includes wt (n = 10), all pax3/pax7 double mutant combinations (pax3a−/−/3b−/− [n = 7], pax3a−/−/7a−/− [n = 5], pax3a−/−/7b−/− [n = 5], pax3b−/−/7a−/− [n = 5], pax3b−/−/7b−/− [n = 5], and pax7a−/−/7b−/−- [n = 6]) as well as pax3b−/−/7a−/−/7b−/− triple mutant embryos (n = 11). B, Embryos in group B can move their pectoral fins both away and toward the body; however, they are unable to move their pectoral fin in an angle >90° away from the body, the triple mutant pax3a−/−/7a−/−/7b−/− (n = 5) embryos belong to group B. C, Group C represents embryos that only can move their pectoral fin toward the body and are unable to flex the fin in an angle >90° away from the body. This group comprises the triple mutant pax3a−/−/3b−/−/7a−/− (n = 10) and pax3a−/−/3b−/−/7b−/− (n = 5) embryos. D, Group D represents embryos that are unable to move their pectoral fin in any direction. This group includes the pax3a−/−/3b−/−/7a−/−/7b−/− (n = 5) quadruple zebrafish embryos. E, Snapshots representing the maximum flexed fin movement for each group is presented. pax7a and pax7b mutant alleles are highlighted in blue and pax3a and pax3b in red. Dotted line represents the 90° angle from the body axis. Arrows indicate movement of pectoral fin