Figure 3

The relative expression levels of Ma_or114-1a and Ma_or114-1b in different male and female individuals of Misgurnus anguillicaudautus, based on the relative quantitative results of qRT-PCR (2^–∆∆CT). Three technical replicates for each individual and each gene, and the error bar is the standard deviation of the three technical replicates. The sample numbers are as shown in Table 1. When comparing expression difference of Ma_or114-1a between the male group and the female group, F = 0.04824, P = 0.09203 > 0.05; t = −2.41623, P = 0.03653 < 0.05 (equal variance assumed); significant. When comparing expression difference of Ma_or114-1b between the male group and the female group, F = 1.123, P = 0.94206 > 0.05; t = −1.37163, P = 0.12104 > 0.05 (equal variance assumed), not significant.