Fig. 5.

Effects on T-cell homeostasis upon PFOS exposure are neutrophil dependent in colitic mice. (A) Experimental outline. Mice received a single dose of TNBS (2.5%, in 50% ethanol, intrarectally administered), five doses of PFOS (10 mg/kg/total dose, orally gavaged) and 500 µg per dose of anti-Ly6G monoclonal antibody every other day. (B,C) Violin plots showing the absolute numbers of CD45⁺ (B) and CD4⁺ (C) T cells in spleen analyzed by flow cytometry. n=3-8, four experiments. (D-G) Flow cytometry analysis of FOXP3⁺ (D,E) and RORγt⁺ (F,G) CD4⁺ T cells in the spleen, following neutrophil depletion. Violin plots represent the absolute numbers and frequencies of these populations out of CD45⁺ cells. n=3-8, four experiments. The black line represents the median. NS, not significant; *P<0.05, **P<0.01, ***P<0.001. One-way ANOVA with Fisher's LSD test.