ZFIN Image: Ahmad-Mansour et al., 2021, Figure 2

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Figure 2

S. pettenkoferi SP165 survival in infected macrophages. S. pettenkoferi SP165 (blue) and S. aureus SA564 (grey) bacteria were used to infect RAW 264.7 (A) or THP-1 (B) macrophages. The amount of bacteria after 5 and 24 h pGt was evaluated after infection at a multiplicity of infection (MOI) of 20. The average and standard deviation (SD) of five different experiments are represented. (C) LDH release was measured using the CyQUANT assay kit after RAW 264.7 macrophage cells were infected at a MOI of 20 for 5 and 24 h, with the exception that the cells were seeded in a 96-well plate and that the extracellular bacteria were not eliminated. Data are expressed relative to the 100% positive control (n = 3 biological repeats). A two-way ANOVA test was used to establish statistical significance, with **** p < 0.0001; ns, not significant.

Acknowledgments

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