Small heads in the trrap-zebrafish mutant zebrafish. (a) Wild-type fish (trrap+/+) at 3 dpf. (b) trrap mutants (trrap−/−) at 3 dpf. Scale bar, 200 μm. (c) The sizes of the head excluding the eyes (dashed line) in the wild-type (trrap+/+: n = 1; trrap+/−: n = 7) and trrap mutant (trrap−/−: n = 8) zebrafish were measured. The error bars indicate the standard deviation. Asterisks indicate statistical significance between the wild-type and mutant zebrafish. ****P < 0.0001. (d) The distance between the eyes (double arrow) in the wild-type (trrap+/+: n = 1; trrap+/−: n = 7) and trrap mutant (trrap−/−: n = 8) zebrafish were measured. The error bars indicate the standard deviation; ns, not significant.
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