Fig. 2 In situ hybridization on wild type and plna R14del mutant adult zebrafish hearts, 2 years of age. A, B Expression of tbx18 to indicate the epicardial cells. C, D Expression of myl7 to indicate the myocardial cells. E, F Expression of grn1 to indicate the immune cells. G, H Expression of postnb to indicate fibroblasts. All stainings were performed on WT n = 3, plna R14del n = 3, two experimental replicates. Zoom-in of each region is indicated. Images were taken at a magnification of ×20. Scale bars are 200 µm for whole-heart tile scans and 50 µm for zoom-in regions. A atrium, V ventricle, BA bulbus arteriosus.
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