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Fig. 2 TgBAC(ankrd1a:EGFP) expression is detected in the trunk and head slow muscle cells. 96 hpf larvae were immunostained with anti-EGFP (cyan) and F310 (fast muscle fibers, magenta) antibodies. (A-A??) Confocal scans of the trunk region shown as separate channels (A, A?) and merged image (A??). (B) 3D reconstruction of the trunk area. (C, D) Lateral (C) and ventral (D) views of the head. Arrows on C and D point to positions of optical cross sections of specified muscles, shown in E-H and I-L respectively. (E-L) Optical cross sections of individual head muscles, designated as: lap = levator arcus palatini, do = dilator operculi, ao = adductor operculi, bl5 = branchial levator 5, imp = intermandibularis posterior, am = adductor mandibulae, ih = interhyoideus and sh = sternohyoideus. In panels E-L, arrows point to regions of signal overlap (yellow color). Scale bars, 100 µm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Reprinted from Gene, 792, Boskovic, S., Marin Juez, R., Stamenkovic, N., Radojkovic, D., Yr Stainier, D., Kojic, S., The stress responsive gene ankrd1a is dynamically regulated during skeletal muscle development and upregulated following cardiac injury in border zone cardiomyocytes in adult zebrafish, 145725, Copyright (2021) with permission from Elsevier. Full text @ Gene