FIGURE 3

Purification and characterization of PcActx peptides. (A). Analytical RP-HPLC chromatograph for the final purified linear PcActx peptide with absorbance at 220 nm with purity ≥90%. (B). Analytical RP-HPLC chromatograph for the final purified oxidized PcActx peptide with absorbance at 220 nm with purity ≥90%. (C). ESI-MS analysis of linear PcActx peptide. Anal. calcd for C₁₈₂H₂₆₅N₅₃O₅₁S₃ [M + H]⁺ 4104.89 Da (monoisotopic mass), found [M + H]⁺ 4104.90 Da. (D). ESI-MS analysis of oxidized PcActx peptide. Anal. calcd for C₁₈₂H₂₆₃N₅₃O₅₁S₃ [M + H]⁺ 4102.87 Da (monoisotopic mass), found [M + H]⁺ 4102.88 Da. (E). ESI-MS analysis of oxidized PcActx peptide incubated in dd-H₂O for 48 h.