ZFIN Image: Rondelli et al., 2021, Figure 3

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Figure 3

CLPX regulates the stability of ALAS2 in MEL cells.A, i, Alas2 mRNA expression was decreased or unaltered in the absence of CLPX and CLPP, (ii) but its protein levels are increased in Clpx^−/− and Clpp^−/− cells. The proteolytically cleaved, mitochondrial matrix localizing form of ALAS2 (38, 39, 40, 41, 42), which interacts with CLPX and catalyzes ALA formation (16), is marked with an asterisk (N = 3). B, ALAS2 stability is increased in both Clpx^−/− and Clpp^−/− cells. This is quantitated in (i), data shown relative to HSP60 expression. ∗p < 0.05. Error bars denote SEM (N = 4). Representative gels are shown in (ii).

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