FIGURE 3
Early light (EL) and early dark (ED) gene expression of insulin-like factors (igf1a and igf1b), and their receptors (igf1ra and igf1rb) in adult Danio rerio. (A) Liver igf1a; (B) muscle igf1a; (C) liver igf1b; (D) muscle igf1b; (E) liver igf1ra; (F) muscle igf1ra; (G) liver igf1rb; (H) muscle igf1rb. The gene expression was normalized by the 18S rRNA and expressed relative to the lowest mean of the control temperature group. The values are expressed as mean ± SEM. n = 3–6 (pools of four animals each). Significant differences are shown when p < 0.05. Red symbol corresponds to 28oC and blue symbol corresponds to 23oC.