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Figure 1
Administration of the isolated bacteria YC and its protective effect on the resistance against A. hydrophila of zebrafish. (A) Phylogenetic tree of the isolated bacteria bacterium. Distance was calculated by using 16S rRNA gene based on the neighbor-joining criterion and the bootstrap confidence values were 100 replicates. (B) Confocal fluorescence imaging of zebrafish larvae (4 dpf) immersed in water containing 106 CFU/ml P. pentosaceus YC (pre-treated with 300 µM TAMRA) for 24 h. (C) Experimental design for accessing the protective effect of P. pentosaceus YC administration. YC was administrated into water at a concentration of 106 CFU/ml for 1 week (YC1W), 2 weeks (YC2W), 3 weeks (YC3W), and 4 weeks (YC4W). PBS treatment as control group (CON). YC or PBS were provided every 24 h for different durations. (D) Survival curve of zebrafish from different groups following 108 CFU/ml A. hydrophila challenge. Each curve represents the sum of three independent tanks. ***p < 0.001 by Mantel–Cox test.