|
Figure 7
Zebrafish BIRC2 colocalizes and interacts with p53. (A) Immunofluorescence analysis of zebrafish BIRC2 and p53 in EPC cells. EPC cells were transfected with the indicated plasmids and then infected with E. piscicida. At 6 hpi, the infected cells were fixed with 4% formaldehyde for 15 min and subjected to immunofluorescence microscopy assays. Scale bar: 10 ?m. (B) The interaction between zebrafish BIRC2 and p53. EPC cells were transfected with the indicated plasmids, then lysed and immunoprecipitated using Anti-FLAG M2-Agarose Affinity Gel beads. The input proteins and precipitates were subjected to Western Blotting using anti-FLAG and anti-pTurboGFP antibodies.