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FIGURE 4
The movements of atrn–/– larvae are faster than the wild type due to higher testosterone concentration at 6 dpf, and human ATRN can rescue the phenotype of zebrafish atrn–/–. (A) The tracking pattern of the wild-type larvae. (B) The tracking pattern of the MZ atrn–/– larvae. (C) Average swimming speed–time graph of atrn WT and MZmut larvae of 144 hpf in 10 min. (D) The average velocity of 24 WT and MZ atrn–/– larvae during 10 min. Error bars, SEM. (E) qRT-PCR results indicate that the expression level of testosterone synthesis key gene hsd17b7 in MZ atrn–/– larvae is increased. (F) Testosterone ELISA assay results show that the content of testosterone in the MZ atrn–/– is also increased. N = 10. (G) qRT-PCR results indicate that the expression level of cyp51 and hsd17b7 in treated MZmut larvae was rescued. (H) Average swimming speed–time graph of 6 dpf larvae injected with hATRN mRNA in 10 min. Error bars, SEM. (I) The average velocity of the control and treated larvae during 10 min. (J) Testosterone ELISA assay results show that the content of testosterone in larvae injected with hATRN mRNA was rescued. N = 20. Data are shown as mean ± SD; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, no significance (p > 0.05).