|
Figure 3
Asb2 is specifically expressed in the cardiac region during early embryogenesis of mice. Whole mount in situ hybridization was performed using murine embryos. (a) Asb2 expression was detected in the cardiac region as early as formation of the cardiac crescent at E7.5. Thereafter, Asb2 expression was localized at the cardiac region until cardiac formation was completed. (b) At E9.5, Asb2 expression was detected in the ventricular myocardium and outflow and inflow tracts. Additionally, extra-cardiac expression of Asb2 was initiated at several somites at this stage. EHF, early head fold; Cc, cardiac crescent; OFT, outflow tract; IFT, inflow tract; v, ventricle; s, somite.