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Fig. 4 Effect of CPBMF-223 (50–500 µM) acute treatment in early stages of heartbeat, exploratory behavior, and neurotoxicity in zebrafish larvae. The heartbeat was evaluated in 2 and 5 days postfertilization (dpf) (A and B), while distance (C), velocity (D), angular velocity (E) and frequency outside area (F) were evaluated in 5 dpf. Data are expressed mean ± standard error of the mean from 18 (Heartbeat) or 30 animals tested individually for each group and were analyzed by one-way analysis of variance, followed by Tukey post-test. *p < 0.05, **p < 0.01, and ***p < 0.001 significantly from vehicle group (negative control).