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Figure 1
Experiments in Tg mice and WT controls. Standard histology of testis (a–d) shows no difference in number, size, and cellular compound of seminiferous tubules between Tg mice and control. Diameter (e), and thickness (f) of 100 seminiferous tubules per mouse were measured: histograms show mean and standard deviation, no significant difference was found; (g) testis weight; (h) sperm count; (i,j) protein and mRNA levels of Dyrk1A in testis and brain; (k,f,l) IHC assay for Dyrk1A in the testis of Tg mice and WT controls. The two groups of mice did not differ in the number and intensity of Dyrk1a+ cells in seminiferous tubules. In control mice, Dyrk1a was expressed by cells close to the basal membrane, corresponding to the early stages of spermatogenesis and as show in the cropped images: double arrowheads: small cells close to the basal membrane, corresponding to spermatogonia; single arrowheads: large cells not in contact with the basal membrane, corresponding to primary spermatocytes; square brackets: later stages of spermatogenesis, with a lower Dyrk1a intensity. p value : 0.033 (*), 0.002 (**), <0.001 (***).