Figure 3

Figure 3

HIVEP1 deficient human monocytic cells show increased NFκB activity and proinflammatory cytokine production upon stimulation with TLR ligands or bacteria. HIVEP1 deficient and control human monocytic THP1-MD2-CD14 cells were stimulated with PAM3CSK4 (1 µg/mL), LPS (100 ng/mL), heat-killed E. coli, S. pneumoniae, K. pneumoniae or P. aeruginosa. HIVEP1 deficiency was associated with (A) enhanced NFκB activity (24-hour stimulation), (B–D) enhanced TNF-α, IL-6 and IL-8 release (4 and 24 hour stimulation) and (E–I) increased IL-1β, TNF-α, IL-6, IL-8 and A20 mRNA expression (normalized to HPRT mRNA). Data shows means ± SEM and were pooled from two (E–I) and three (A–D) independent data set (n = 4 for each condition within each experiment). *P < 0.05, **P < 0.01, ***P < 0.001.